Cât timp se poate păstra o soluție injectabilă trasă într-o seringă?

[dnBQByvHb8Zkawbjpx]

Dozele mici (<100 mg) sunt în mod notoriu dificil de cântărit cu precizie, cu excepția cazului în care se utilizează cântare de foarte mare precizie. De exemplu, pentru a pregăti ketamina pentru IM, poate fi mai bine să se pregătească un lot de cinci injecții prin dizolvarea a 500 mg K în 5 ml de soluție salină, în loc să se cântărească 100 mg pentru o singură injecție (care ar putea fi de 150 mg din cauza erorii cântarului).

Luați în considerare o soluție sterilă garantată preparată prin fierberea K în soluție salină. Soluția este apoi trasă în seringi sterile folosind ace sterile noi sau filtre de seringă de 0,22 um. Acul fiecărei seringi este apoi înlocuit cu unul nou. Cât timp se poate păstra în siguranță o astfel de injecție preparată, probabil în frigider la 4-5 °C? Aspectul stabilității K nu mă preocupă, deoarece există studii care nu au arătat nicio degradare a K depozitat în seringi. Mă îngrijorează siguranța microbacteriană a injecțiilor depozitate după o săptămână sau maxim două săptămâni. Ar putea fi utilizate în siguranță?

 

[Paracelsus]

În timp ce stabilitatea chimică a ketaminei în soluție este bine stabilită, problema principală este potențialul de contaminare bacteriană în timp, în special atunci când seringile preparate sunt depozitate în frigider.

Considerații privind sterilitatea:

1. Preparare sterilă: Utilizarea filtrelor de seringă de 0,22 µm reduce semnificativ riscul de contaminare bacteriană, deoarece acestea sunt concepute pentru a elimina majoritatea bacteriilor. Cu toate acestea, este posibil ca unii viruși și particule mai mici, inclusiv sporii, să nu fie complet filtrați, deși acesta este un risc relativ scăzut.
2. Depozitarea sterilă: Odată ce soluția este preparată și seringile sunt umplute, înlocuirea acului cu unul steril nou contribuie la menținerea sterilității. Asigurarea că seringile și acele utilizate sunt sterile individual și manipulate într-un mediu curat minimizează și mai mult riscurile.
3. Refrigerare: Păstrarea seringilor preparate în frigider (la 4-5°C) încetinește dezvoltarea bacteriană, dar nu o oprește complet. Refrigerarea este eficientă în prelungirea sterilității soluțiilor injectabile pentru o perioadă limitată, deoarece majoritatea bacteriilor și ciupercilor se dezvoltă mai lent la temperaturi scăzute. Cu toate acestea, nu este infailibilă împotriva contaminării, în special pe perioade îndelungate.

Siguranța microbiană a injecțiilor depozitate:

1. Prima săptămână: Dacă soluția este preparată în condiții sterile optime și depozitată în frigider, se consideră, în general, că poate fi utilizată în siguranță timp de până la o săptămână. Aceasta se încadrează în intervalul în care riscul de contaminare bacteriană rămâne scăzut, presupunând că nu există încălcări ale sterilității în timpul preparării sau depozitării.
2. A doua săptămână: Extinderea depozitării la două săptămâni crește riscul de contaminare bacteriană. Deși refrigerarea și utilizarea de echipamente sterile atenuează acest risc, unele organisme oportuniste se pot multiplica lent în condiții de refrigerare. Riscul este mai mare după prima săptămână, astfel încât, dincolo de aceasta, siguranța soluției devine mai discutabilă.

Continuați să utilizați echipamente sterile (seringi, ace, soluție salină și filtre) și mențineți condiții de lucru curate în timpul preparării. Etichetați fiecare seringă cu data preparării pentru a asigura utilizarea în siguranță în intervalul de timp recomandat.

 

[dnBQByvHb8Zkawbjpx]

Vă mulțumesc pentru comentariul detaliat. M-am gândit în același sens: filtrarea nu elimină virușii (prea mici), motiv pentru care optez pentru sterilizarea prin căldură la 99 °C ca metodă principală care sterilizează soluția.

Intenționez să prepar loturi de maximum cinci shot-uri la un moment dat; unul dintre ele ar fi consumat imediat și până la patru ar fi depozitate. Ultima dintre ele ar fi utilizată la începutul celei de-a doua săptămâni și mă bucur să aud că planul meu nu este scandalos de prost și/sau periculos.

Nu am avut etichetarea fiecărei seringi în protocolul meu și cu siguranță voi face asta, vă mulțumesc pentru că ați subliniat acest lucru.

 

[SoldadoDeDrogas]

I used to do something similar. I would prep a bunch of shots and leave them in a sunglasses case. If you are worried about bacterial growth, perhaps you can just cook it and heat the needle tip right before use?

There was another benefit, I don't know if it applies in EU or where else. In the USA though, if you get caught with a syringe loaded with drugs, they cannot charge you with possession. Some people used to load up all their stuff and carry it around like that.

 

[dnBQByvHb8Zkawbjpx]

We are not on the same page. See this picture how needles are packed. They are individually packed in a sterile pouch, and each needle is under a protective plastic cover so it can't hurt anyone before the actual administration. Unpacked and immediately put on a new syringe I am not concerned about the bacterial safety here. Heating needles would do more harm than good and is absolutely unnecessary.

My only concern is about the drug itself since you never know what hands touched it and how it's contaminated. Syringes, needles, saline, these are all sterile.

 

[SoldadoDeDrogas]

Yes, obviously, my friend. I don't expect you are talking about discount syringes from an open box at the thrift shop. You have to open it and expose it to contamination in order to get the substance into it, though, yes?
Just boil the ketamine solution and load them up. It isn't just the product but the handling and procedure and everything. If a spore syringe can survive, this should have better odds.
The drug itself is probably antiseptic also in high concentration, all the germs would OD

 

[Osmosis Vanderwaal]

99c isn't hot enough to sterilize. It barely sanatizes. Normally one would call that an ultra-pasteurization, thich is the worst place to be because tou've killed any actinomycetes or helpful bacteria that could have protected your whatever, but you didn't get it hot enough to kill thermophiles are endospores. When you don't have labratoy equipment, working between vials with injection ports and syringes is the most sanatar method by about 100 light years. Remember, if you want to keep your vials sanatary, flame is the most effective antiseptic in the world. A split second at 1000 degrees kills basically everything . Flame thos syringe needles before you stick them in the ports
>100mg is a low dose? Half that is more than I take at a time.
You'll be hard pressed to gind a fungal basidiospore or ascospore smaller than 0.22. You won't. They usually start at 20x that size. Bacteria is by and large much bigger even. Viruses, now you ain't going to filter a virus out.
One thing to consider if you are going this route is spring water, ro filtered water, drinking water, they all contain bacteria. Reverse osmosis doesn't remove bacteria. Distilled water is the only way to go. Some bacteria can survive on the trace elements in spring water. One more thing. As a pormer lab tech, the #1 source of contamination is you. Clean out from under your fingernails. Cut them short. Wear gloves. Wear a mask to at least diffuse the bacteria spewing from your mouth. Be aware of your mouth. Keep it closeed, it might as well be a toilet,.

 

[dnBQByvHb8Zkawbjpx]

All used injection material is purchased through pharmacies, packed individually and sterile, relying on reputable brands (BD, Terumo). There is no need to sterilize any such material. Needles are under a plastic cover even after they are removed from their pouch and it would be silly to sterilize them with fire.

I use sterile physiological solution (0.9 % NaCl in H2O, sterile, packed 5 ml per vial) so the only non-sterile substance in the whole process is the drug itself, ketamine in my case. I thought I could bake it to sterilize, then load portions into syringes and refrigerate them until use (no more than 2 weeks). Or I could dissolve K in the solution and boil for a bit to kill any pathogens, then load into syringes. Or I could simply not bother and hope that it has not been contaminated too much and let my immune system handle whatever may get through.

You make good points about protective equipment to limit self-contamination during the process. I will keep those in mind. Coincidentally the original post and the answers are only becoming relevant to me in present time and just in time. Sometimes I like to plan ahead as was the case of this post made 8 months ago.

 

[SoldadoDeDrogas]

When I first started experimenting with syringes it was IM ketamine from liquid vials from the veterinarian. I've shot all types of shit off the street since then, sometimes without much care. It's been about 20 years and the worst thing that has happened was my hand/wrist inflammed badly one time with an infection. I was shooting meth and I was out on the street for about a week.. fun times. That was arguably just my own lack of hygiene or circumstances at the time. Some antibiotics and it went away. I have been pretty careless and reckless towards myself, by my own and definitely by most peoples standards, and am probably more lucky than anything, It all just kinda common sense, you know? That is just to provide an example of what you can get away with, I guess. It is not something to fool around with, but it is not so delicate either. I think you have enough sense.

Anyway, if you do everything proper when prepping them and store them in the fridge like you mention, I would think you would be able to keep them for a while.... months... longer even, without any problem. What about if you added some vinegar or something consumable with antibiotic properties to that end, to keep growth at bay? With all the above prep you mention, with added antibacterial and reheating it before use, I don't see it ever being an issue.

 

[dnBQByvHb8Zkawbjpx]

I am not going to contaminate intramuscular injectables with vinegar or any other home grown bacterial retardant. Working towards the sterility of the shot is much easier for me and does not add any undesired substances.

 

[mycelium]

In prison we used vinegar, stores in a non sterile medicine bottle, to draw vinegar into an old syringe, and squirt into crack, so we could break it down and shoot it.
Just sayin.
I have sharpened a syringe with matchbooks and 2 pennies to remove any burrs.
Little bleach to clean everything before and after...
Good thing I'm not stupid anymore

 

[SoldadoDeDrogas]

I mean, if it is that complicated, just cook your shots as needed like everyone else. Or don't.
Whatever you wanna do. It is great to be cautious and everything, but you are over thinking it a bit. We are just suggesting ideas and giving examples, noone is telling you to mainline old pickle juice.
Seems like communicating with understanding is too much work with some folks.

If I have to go back and review parts of my life it will be rather embarrassing at times, for sure. I can't even wrap my mind around that one, though, shooting crack in jail? By itself? That's probably one of the last things I would ever want to do.

Unrelated topic, I apologize, but what about using these syringe filters to make spore syringes?

 

[mycelium]

The syring filters are specifically designed to not let spores pass, as almost no spores pass below the .2 micron size of the filter.

And someone else wanted to shoot coke, but there was none available, so he got crack and I showed him how to break it down...and I got half....
And then he went back and got more....
Personally I would have rather him spent the money on an oz of weed, or even pharmaceuticals, but he likes shooting coca...at the time, I would not turn down free drugs

 

[mycelium]

And I didn't say it was old pickle juice I said it was vinegar, which is dilute acetic acid, which is just backwards from making crack, it's a base to acid reaction.
And I was saying that bleach kills bacteria/viruses, and if it will kill aids, then it's sure to kill refrigerator bacteria(maybe lactobacillus/bacillus/pseudomona(spelling)....

 

[SoldadoDeDrogas]

I know buddy, I didn't mean you. The first paragraph was for the thread author, I should have specificied, but I thought I was being a little redundant, my apologies.

 

[Osmosis Vanderwaal]

Ketamine hcl melts at a lower temp. rthan even crack, 93c or something, has a ph of about 5.5 in an injectable solution.....I don't think heat sterilization is going to work and the ph isn't enough to hamper bacteria. Most of the time, what happens is, the thing that happens most often, nothing ever happens. If someone asked me and were immunocomprimised or otherwise paranoid, there is at least 1 good solution. 0.022 um syringe filter. About a buck and can be reused and it;ll fix you right up for anything but virus or mycoplasma

 

[mycelium]

And when they make syringes and other medical things, they don't use an autoclave , they use UV-C light, and gamma radiation...I found a hundred dollar UV-C wand on scamazon, and couldn't find a gamma device, for sterilizing mushroom stuff, but I got sidetracked and looked for something else

 

[Osmosis Vanderwaal]

Right UV is an obvious choice. It doesn't penetrate a substrate butl liquids especially, are easily sterilized. I once upon a time worked for a city ( A ccouple , at different times) and amoung other things, cleaning the uv lights in the effluent chute was part of the job. The sewage came into a pond with several aerators, the solids sunk to the bortom and the sewage was skimmed off the top and passed under uv lights and into the river ir went.